Fig. 1. Effect of quercetin on adipose inflammatory responses in HFD-fed mice. Levels of inflammatory protein (A) TNF-α, (B) IL-6, (C) MCP-1, and (D) MIP-1α in adipose tissue from mice fed either a regular diet (RD), a high-fat diet (HFD) or a high-fat diet containing 0.05% quercetin (HFD+Que) for 9 weeks. Adipose tissue (200 mg) was homogenized with 400 ?L of 100 mM Tris-HCl and 250 mM sucrose buffer (pH 7.4) supplemented with protease inhibitors. Lipids were removed by centrifugation at 10,000 g for 10 minutes. Levels of cytokine in homogenates were measured by enzyme-linked immunosorbent assay and normalized for protein. Results are means±SEM. *P<0.05, †P<0.01, ‡P<0.001 compared with obese mice fed on HFD.
Korean J Obes 2014;23:170~178
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