Fig. 1. Experimental scheme. For the induction of adipocyte differentiation, 3T3-L1 preadipocytes were seeded. At confluence (day 0), the cultured preadipocytes were induced to differentiate by the addition of differentiating medium containing 115 μg/mL methylisobutylxanthine (IBMX), 10 μg/mL insulin, and 1 μM dexamethasone (Dex) from day 0 to day 6. At day 6, medium was changed with medium containing 10 μg/mL insulin for an additional 6 days from day 6 to day 12. The medium was refreshed every 3 days. At day 12, differentiated 3T3-L1 cells were subjected to Oil red O solution or used for Western blot analysis. Zaluzanin C was added to the cell culture medium at concentrations of 0, 1, 2.5, 5, and 10 μM from day 0 to day 12. DMEM, Dulbecco’s modified Eagle’s medium.
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