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Fig. 4. Effect of zaluzanin C on the expression of peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid binding protein 4 (FABP4) and CCAAT/enhancer binding protein alpha (C/EBPα) in 3T3-L1 cells. 3T3-L1 cells were treated with differentiation medium in the absence or presence of different concentrations of zaluzanin C (0, 1, 2.5, 5, 10 μM) for 12 days. From each whole cell lysate, 10 μg of proteins was resolved by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis for determination of FABP4, PPARγ and C/EBPα expression. β-actin expression is shown as a loading control. The bands (A) were quantified using image analysis software, and their relative intensities of PPARγ (B), FABP4 (C), or C/EBPα (D) were expressed as target protein/β-actin. The results are presented as mean±standard error of the mean of three independent experiments (n=3). *,†,‡Indicate statistically significant differences between groups.
J Obes Metab Syndr 2019;28:105~111 https://doi.org/10.7570/jomes.2019.28.2.105