Fig. 3. Water extract of Artemisia annua (WEAA) increases uncoupling protein 1 (UCP1) mRNA and protein expression in C3H10T1/2 cells. (A) UCP1 gene expression, as evaluated by real-time polymerase chain reaction. Relative mRNA expression was normalized to 36B4 gene expression. (B) UCP1 protein expression, as detected by Western blotting. β-actin was used as a loading control. Band intensity was calculated using ImageJ software. The results are representative of at least three independent experiments. Values are presented as mean± standard deviation and are significantly different from the WEAA-untreated positive controls, *P < 0.05; †P < 0.001. Conc, concentration.
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