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Fig. 5. Water extract of Artemisia annua (WEAA) suppresses body weight gain and decreases fat tissue weight and adipocyte size in Sprague Dawley (SD) rats with high-fat diet (HFD)-induced obesity. SD rats were orally administered WEAA (200 mg/kg) or water once a day for 7 weeks. SD rats were fed a normal diet or a 60% HFD during the experimental period. (A) Body weight, measured weekly. The experimental groups are as follows: (1) SD vehicle group with a normal diet (ND+water, n= 9), (2) SD vehicle group with a HFD (HFD+water, n= 9), and (3) SD rats with a HFD+200 mg/kg WEAA group (HFD+WEAA, n= 9). (B, C) The micro-computed tomography (CT) image is representative of each experimental group and fat volume was calculated using the micro- CT software. (D) Relative organ weight, calculated as percent of organ weight/body weight. (E) Adipocyte diameter, calculated by measuring at least five adipocyte globules in each abdominal tissue of the experimental group. H&E images are representative of each group (× 100). Scale bars indicate 50 μm. Values are presented as mean± standard deviation (n = 9). All statistical analyses are calculated compared to the HFD+water group. (A, C) Two-way analysis of variance (ANOVA) followed by the Bonferroni post-test was used; (D, E) One-way ANOVA followed by Dunnett post-test was used. *P < 0.05; P < 0.01; P < 0.001.
J Obes Metab Syndr 2021;30:32~43 https://doi.org/10.7570/jomes20097