J Obes Metab Syndr 2022; 31(1): 92-93
Published online March 30, 2022 https://doi.org/10.7570/jomes22017
Copyright © Korean Society for the Study of Obesity.
Eun-Yong Choi1, Chan Young Park1, Seong Hyun Ho2, Su-Jin Park2, Donghyun Kim3,4, Byoungduck Han5, Seon-Hee Kim1
1Sungkyun Biotech Co., Ltd., Suwon; 2G&P Bioscience Co. LTD., Goyang; Departments of 3Biomedical Sciences and 4Microbiology and Immunology, Seoul National University College of Medicine, Seoul; 5Department of Family Medicine, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Updates have been made to the Abstract (p. 32) and Methods (p. 34). The Results in abstract has been revised to show the sex and the entire number of experimental animals. The reason why male rats were selected for the current study has been explained in Methods. The original version of the article can be found at https://doi.org/10.7570/jomes20097.
The Methods of the abstract has been revised to read:
The subsection of “Animals and experimental groups” in the Methods has been revised to read:
Zucker lean rats (Lean fa/+) and Zucker fatty (ZF) rats (fa/fa) (male, 5 weeks) were purchased from RaonBio (Yongin, Korea). The experimental groups were as follows: (1) a Zucker lean vehicle group (ZL, n=5); (2) a ZF vehicle group (ZF, n=5); and (3) a ZF+50 mg/kg WEAA group (ZF+WEAA, n=5). Food (Teklad certified global 18% protein rodent diet 2918C; Teklad, Envigo, Indianapolis, IN, USA) and water were provided ad libitum. Oral administration of vehicle or WEAA was started on the day after separation into groups and regularly performed once a day for 11 weeks. Body weight and food intake were checked once a week. Body fat mass was measured using dual energy X-ray absorptiometry (InAlyzer; Medikors, Hovedstaden, Denmark) after the animals were anesthetized with isoflurane. Fat in tissue was calculated as follows.
Fat in tissue (%)=fat mass/(total body mass–bone mineral content)×100
In addition, the organs were extracted and their weights were measured. This study was performed with approval by the Animal Research Ethics Committee of KPClab (approval No. P182016).
SD rats (male, 5 weeks old) were purchased from Orientbio (Seongnam, Korea). After 1 week of acclimation, SD rats were randomly divided into three groups (n=9) in individual cages. The experimental groups were as follows: (1) a SD vehicle group with a normal diet (ND+water, n=9); (2) SD vehicle group with a HFD+ water, (n=9); and (3) SD rats with a high fat diet+200 mg/kg extract of
For all experiments, males were selected for excluding the effect of menstrual cycle.